sigV [2019-01-04 13:22:23]

[SW|RNA polymerase] ECF-type [SW|sigma factor] SigV, required for resistance to lytic enzymes

locus: BSU27120

pI: 9.04

mw: 19.57 kDa

protein length: 166 aa

gene length: 498 bp

function: resistance to lytic enzymes

product: [SW|RNA polymerase] ECF-type [SW|sigma factor] SigV

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 2,769,850 → 2,770,350

Phenotypes of a mutant

• increased sensitivity to lysozyme, this can be suppressed by overexpression of'' [gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]'' [Pubmed|21856855]

The protein

Protein family

• ECF subfamily (according to Swiss-Prot)

Effectors of protein activity

• activity is controlled by the interaction with [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|RsiV], proteolytic degradation of [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|RsiV] releases active [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV] [Pubmed|23687273]

Structure

• [PDB|5WUQ] (the [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]-[protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|RsiW] complex, 28% identity for [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV])

Additional information

• Expression of the [SW|SigV regulon] in increased in ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutants [Pubmed|22362028]

Expression and Regulation

Operons

• genes: [gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]-[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]-[gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]-[gene|FC37C7A0C0D9F50CA1D44B8D939CF06E210D4B38|yrhK]
  description: [Pubmed|21856855]

  sigma factors

  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, [Pubmed|21856855], in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]

  regulation

  • induced by lysozyme ([protein|search|SigV]) [Pubmed|21856855]

  additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]

Biological materials

Mutant

• MGNA-A144 (sigV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/144 NBRP B. subtilis, Japan]
• 1A907 ( ''sigV''::''kan''), [Pubmed|15838020], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A907&Search=1A907 BGSC]
• BKE27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC
• BKK27120 (Δ[gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCAATAAAGGGCTCCT, downstream forward: _UP4_TTGACGAAGGAGGATCTTTC

labs

• [SW|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas Wiegert-Dateien/Thomas Wiegert.html Homepage]

References

Reviews

• 24921931,26901131

Original publications

• 14993308,16274938,17675383,19745567,22362028,23687273,20817771,21926231,21856855,26399770,29069433,30020925